ImageXpress微共聚焦高含量成像系统
Leszek Kotula, M.D., Ph.D., 315-464-1690, [email protected]
这种多功能成像系统具有独特的共聚焦技术, which allows you to explore more physiologically, relevant, complex three dimensional models, including spheroids, tissues, 和整个生物体，并以高通量为载玻片或1至1536孔微孔板上的样品生成出版物质量的图像. 该装置的主要特点是机载机器人流控头, including the following specifications: automated liquid handling for compound/media addition; ability to perform well washing using disposable pipette tips; add, wash, and dispense into user configurable patterns; fully customized workflow through scripting; single-channel pipettor with dispense volumes from 3 µL to 200 µL (±1 µL ±5%); supports 96- or 384-well format pipette tips; holds two temperature-controlled plates for compound addition or media exchange; 3D analysis tools are integrated into acquisition and analysis software and are able to measure volumes, diameters, and distances in 3 dimensions. 此外，软件在8个并行处理器上运行，用于快速多线程分析.
Key capabilities of the microscope:
- 选择最适合您的分析和吞吐量需求的共聚焦几何形状
- 通过我们独特的光路技术，在不牺牲吞吐量的情况下实现出色的图像质量
- 利用先进科学的CMOS探测器快速获取统计相关数据, enabling >3 log dynamic range
- Large field of view enables whole-well imaging
- 通过透射光，液体处理和环境选项扩展您的研究能力
- 用4倍放大倍率的单幅图像捕获384孔板的整个孔
- 在一张4倍放大的图像中捕捉1536孔板的4个孔
- Throughput of >160K wells/day confocal, >200K wells/day widefield 2 ImageXpress
Rules of operation
ImageXpress微共聚焦项目向大学的所有研究者开放, 我们必须执行一些规则，以确保每个调查员都有平等的机会使用我们的设备:
- 所有使用者在使用显微镜之前必须经过培训. 培训结束后，将建立一个账户，用于预留时间和使用显微镜.
- 所有用户必须在网上日历上注册，以便在使用显微镜之前预留时间.
- Reservation policies:
- No reservation more than 2 weeks in advance
- The reservation times may separate to two categories:
- Regular working slots (8am to 5pm, Monday to Friday)
- 非工作时间(周末及平日上午8时前或下午5时后).
- During a regular working slot one user can reserve an instrument for max 4 hours per day and 8 hours per week; however this can be combined with an off-hour slot where the reservation is not limited.
- 如果你不能使用你所预订的显微镜, 请尽可能提前在日历上删除您的预约时间，以便其他人可以看到您的空闲时间.
- 如果您在预定时间结束前完成或在预定时段当天取消, 请通知下一个或前一个排队的人，以便他/她可以早些开始或继续更长时间. 如果您是当天的最后一个用户，并且您在24小时内取消了预订, 你有责任确保关掉显微镜. 在完成后关闭系统之前，请务必通知用户, otherwise he might leave the microscope on for you.
- Using the microscope:
- Please start your session punctually. Repeat "No-shows" will lose their sign-up privileges.
- 请在您的课程结束时查看在线预订日历，以便在最后一刻更改和更新时间表. Leave the system on if someone is reserved next within two hours; keep the laser in standby whenever possible to prolong their life.
- 使用者必须清洁使用过的显微镜物镜，用完后处理垃圾. 如果您携带任何活体标本或潜在有害样品，请将其处理在您的实验室.
- 晚上的最后一个用户负责关闭显微镜系统.
- You are responsible for your data. The system computers are for temporary image storage. 请将您的数据保存在计算机的数据硬盘上，并在获取后尽快传输您的图像数据. 在计算机硬盘驱动器上留下的映像文件将在一段时间内被删除.
- 使用者在使用显微镜时，如有任何问题或损坏，请及时报告.
- No food or drink is allowed in the microscope rooms.
Billing rules:
- The hours billed are based on your computer log off. Remember to log off to end the billing session.
- 所有用户必须在每台显微镜的纸质日志上记录系统使用情况.
- Minimum time billed is 1 hr
- 每次成像将按预留时间或以1小时为单位使用的小时数中较大者计费.e., all time is round up to a full hour)
- 技术支持以1分钟为单位计费，并添加到使用成本/小时中
- 电力用户的年费已被季度折扣所取代. There is now a quarterly cap on the cost of laser use. Other fees (training, 技术支持等)除激光使用外还收费，不打折.
如果你没有在日历上安排的时间出现, you will be billed for the amount of time scheduled.
删除(取消)或修改日程表上安排的时间必须在您安排的时间之前至少24小时完成，否则您将按照最初安排的时间收取费用. (注意日历保留所有条目和修改的电子记录).
Core Fees
How to Access Core Services:
Phone: 315-464-1690
Email: [email protected]